Monolith | 2019-2020

Designers: Stefan Reichl, Andrea Krueger, Martin Jelinek, OXXID GmbH, Munich, Germany
Manufacturer: NanoTemper Technologies GmbH, Munich, Germany

Monolith measures the broadest range of binding affinities in-solution, using a very small sample.

Measure high and low affinities in the same system. Different projects have different demands. It’s inevitable that you’ll need to look at both weak and strong molecular interactions. With a broad sensitivity range, Monolith measures Kd s from pM to mM, so you can tackle as many projects as you want.

Measure in solution, in close-to-native conditions. Immobilizing molecules in SPR assays often causes them to lose activity, and you’re left without results.

Because Monolith measures in solution in most buffers, both binding partners are free to interact in their native conformation, so you’ll finally get results.

Characterize binding events with very little sample. It’s not easy to prepare the large volumes of highly concentrated target and ligand required by ITC.

With Monolith, you get a Kd in a fraction of the volume and concentration, leaving you with more sample for additional experiments.